Biology Protocols

  • Increase font size
  • Default font size
  • Decrease font size

Hogness modified freezing medium (HMFM)

E-mail Print PDF

For storage of cultures at -70˚C.

Recipe:

For 10 X Pre-stock

Constituent 200 ml

K2HPO4

1.254 g
KH2PO4 0.354 g
Na3 citrate 0.952 g
MgSO4.7H2O 0.493 g

For final 1 X HMFM

Dilute 10 X pre-stock 1:10 and add 44 % glycerol:

Constituent 200 ml
10 X pre-stock 20 ml
Glycerol 13.85 g (11 ml)
dH2O 169 ml


Add this to your website