Biology Protocols

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Amido Black Stain (Napthalene Black 12B)

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Used for visualisation of protein on transferred (blotted) onto nitrocellulose membranes.


Consituent 100 ml 500 ml
0.1 % amido black 100 mg 500mg
40 % methanol 40 ml 200 ml
1 % acetic acid 1 ml 5 ml
dH2O to 100 ml to 500 ml


Dissolve the dye in the methanol, then add the acid and make up to the final volume with dH2O

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