Biology Protocols

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CAPS Transfer Buffer (cyclohexylaminopropane sulfonic acid)

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Used for transfer of proteins to nitrocellulose membrane (step of Western blotting).

Recipe:

Consituent 1 L 2 L
CAPS 2.21 g 4.42 g
DTT (optional) 0.5 g 1.0 g
Methanol 150 ml 300 ml
dH2O to 1 L to 2 L

Protocol:

Adjust pH to 10.5 with NaOH and chill to 4˚C.

NOTE: For proteins > 60kDa reduce methanol content to 1 % i.e. for 1 L add 100 ml as opposed to 15 ml.


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