Biology Protocols

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Using the correct precentage acrylamide for SDS-PAGE

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Make sure you are using the correct percentage acrylamide for SDS-PAGE analysis of your proteins of interest.  Detailed in the table below are the effective resolution ranges of commonly cast SDS-PAGE gels.

% acrylamide gel Size Range (kDa)
6 60-200
8 40-140
10 20-80
12 15-70
15 10-50

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