Biology Protocols

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10X Triplex Annealing Buffer

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Triplex DNA can be used as substrates for the analysis of a variety of DNA motors, various triplex forming sequences have been identified and once formed can be exchanged into a desired buffer for analysis/assay.


Consituent For 50 ml
25 mM MES, pH 5.5 1.25 ml (1M Stock)
10 mM MgCl2 0.5 ml (1M Stock)


To anneal, mix equimolar concentrations of linear (dsDNA duplex) and TFO in a 1X concentration of the above buffer.  Heat to 57˚C for 15 minutes and slowly cool to room temperature (preferably overnight).

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