Biology Protocols

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10X Triplex Annealing Buffer

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Triplex DNA can be used as substrates for the analysis of a variety of DNA motors, various triplex forming sequences have been identified and once formed can be exchanged into a desired buffer for analysis/assay.

Recipe:

Consituent For 50 ml
25 mM MES, pH 5.5 1.25 ml (1M Stock)
10 mM MgCl2 0.5 ml (1M Stock)

Protocol:

To anneal, mix equimolar concentrations of linear (dsDNA duplex) and TFO in a 1X concentration of the above buffer.  Heat to 57˚C for 15 minutes and slowly cool to room temperature (preferably overnight).


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