Biology Protocols

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Extraction of DNA from agarose gels using filter tips

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1. Carefully cut the agarose gel slice containing your PCR product (or DNA fragment) as finely as possible so as not to take excess agarose.
 
2. Place the gel slice in the top of a 200 µl filter pipette tip (make sure they are hydrophilic filters).
 
3. Cut the bottom off the tip so that it fits into a 1.5 ml tube.
 
4. Spin at max speed for 30 secs.
 
5. Remove the tip and keep the flow through.

 


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